RNAlysis quick-start video guide
Launching RNAlysis
Open the graphical user interface by running rnalysis-gui in your terminal or executing gui.run_gui() in a Python console.
![_images/load_table.webp](_images/load_table.webp)
Importing Data
Select a file from your computer and click ‘Start’ to import it into RNAlysis.
![_images/load_table.webp](_images/load_table.webp)
Exploring Your Dataset
View an overview of your data, including table name, type, and dimensions. Use the ‘View full table’ button for a comprehensive look.
![_images/view_table.webp](_images/view_table.webp)
Applying Filters
Select a filtering function, adjust parameters, and click ‘Apply’ to modify your table. Changes won’t affect the original file until saved.
![_images/filter_table.webp](_images/filter_table.webp)
Undo the operations you applied to your data
Use the ‘Command history’ window to undo or redo operations applied to your table at any time.
![_images/undo_actions.webp](_images/undo_actions.webp)
Apply your operations ‘in-place’ or make a copy
Apply operations ‘in-place’ or to a copy of your table in a new tab, leaving the original unmodified.
![_images/apply_inplace.webp](_images/apply_inplace.webp)
Generating and Saving Visualizations
Create graphs of your data, resize them, and save in various file formats for further use or presentation.
![_images/generate_graphs.webp](_images/generate_graphs.webp)
Analyze Multiple Tables at the Same Time
Open new tabs to work on multiple tables simultaneously, enhancing your analytical capabilities.
![_images/new_tab.webp](_images/new_tab.webp)
Organizing Your Workspace
Sort tabs and customize their colors to efficiently manage your analytical workspace.
![_images/sort_tabs.webp](_images/sort_tabs.webp)
Saving Modified Tables
Click ‘Save table’ to store the results of your filtering operations in a location of your choice.
![_images/save_table.webp](_images/save_table.webp)
Creating Gene Sets
Import lists of genes as Gene Sets for specialized analysis within RNAlysis.
![_images/import_gene_sets.webp](_images/import_gene_sets.webp)
Visualizing Gene Set Intersections
Use the ‘Visualize Gene Sets’ window to create Venn diagrams and UpSet plots, illustrating intersections between tables and gene sets.
![_images/visualize_gene_sets.webp](_images/visualize_gene_sets.webp)
Performing Set Operations
Extract specific subsets from your data using predefined set operations or by selecting subsets in the preview pane.
![_images/set_operations.webp](_images/set_operations.webp)
Conducting Enrichment Analysis
Perform various types of enrichment analysis on your filtered tables and gene sets to gain biological insights.
![_images/enrichment_analysis.webp](_images/enrichment_analysis.webp)
Rapid Gene Information Lookup
Right-click to quickly access gene information from various biological databases, customizable in the settings menu.
![_images/quick_search.webp](_images/quick_search.webp)
Building Analysis Pipelines
Group multiple operations with specific parameters into a Pipeline to streamline your data analysis workflow.
![_images/create_pipeline.webp](_images/create_pipeline.webp)
Executing Pipelines
Apply Pipelines to multiple tables simultaneously, enhancing efficiency and reducing errors in your analysis.
![_images/apply_pipeline.webp](_images/apply_pipeline.webp)
Integrating External Bioinformatic Tools
Access a graphical interface for various bioinformatic tools, covering stages from adapter trimming to differential expression analysis.
![_images/external_windows.webp](_images/external_windows.webp)
Leveraging Table-Specific Functions
Specify table types when loading to access specialized functions, such as clustering analysis for count matrices.
![_images/table_types.webp](_images/table_types.webp)
Recovering Closed Tabs
Restore accidentally closed tabs through the ‘Edit’ menu to recover your work.
![_images/restore_tabs.webp](_images/restore_tabs.webp)
Generating Interactive Analysis Reports
Create comprehensive, interactive analysis reports with a single click, automatically including all generated tables, gene sets, and graphs.
![_images/create_report.webp](_images/create_report.webp)